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Table 2 Protein data for the 54 protein isoforms identified in this study

From: Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites

Protein name

PlasmoDB ID and NCBI GenBank accession number

Calculated

Spot number

Mascot MS/MS ion search

Average volume ratio

Relative standard deviation

Maximum fold change

One-way ANOVA P value

  

Mass (kDa)

pI

 

Score

Peptides matched

Sequence coverage

TP1

TP2

TP3

TP4

TP1

TP2

TP3

TP4

Median

  

2-Cys peroxiredoxin

[PDB:PF14_0368]

[Genbank:AAN36981]

22.0

6.7

-

559

4

48%

1.03

1.02

0.96

0.78

5.3%

15.4%

9.9%

6.4%

8.1%

1.33×

0.016

Actin-I

[PDB:PFL2215w]

[Genbank:AAN36527]

42.1

5.2

1

533

9

40%

0.58

0.97

1.30

1.24

5.9%

5.9%

8.5%

3.2%

5.9%

2.24×

<0.001

    

2

609

8

37%

0.60

0.93

1.44

1.25

4.8%

5.6%

5.6%

3.4%

5.2%

2.39×

<0.001

    

3

391

8

36%

0.66

1.01

1.60

1.35

7.0%

4.4%

7.0%

2.2%

5.7%

2.43×

<0.001

    

4

415

5

21%

0.63

0.94

1.21

0.97

7.7%

3.4%

8.6%

6.8%

7.3%

1.91×

<0.001

    

5

192

6

29%

1.57

0.73

0.78

1.40

8.7%

7.0%

12.6%

11.3%

10.0%

2.15×

<0.001

Adenosine deaminase

[PDB:PF10_0289]

[Genbank:AAN35486]

42.9

5.4

-

484

7

23%

1.24

1.18

1.15

0.80

14.5%

5.0%

2.6%

6.6%

5.8%

1.55×

<0.001

eIF4A/RNA helicase-1/helicase 45

[PDB:PF14_0655]

[Genbank:AAN37268]

45.3

5.5

1

318

8

24%

0.41

1.32

1.19

1.01

4.4%

3.7%

7.8%

5.8%

5.1%

3.26×

<0.001

    

2

159

2

10%

0.61

1.24

1.23

1.05

5.6%

5.0%

6.6%

2.7%

5.3%

2.03×

<0.001

    

3

143

6

22%

0.73

1.20

1.30

1.16

8.8%

6.1%

20.6%

9.5%

9.2%

1.79×

<0.001

    

4

133

4

19%

1.58

0.57

0.88

1.11

5.7%

5.2%

8.2%

3.6%

5.4%

2.78×

<0.001

    

5

242

4

13%

1.64

0.62

0.94

1.22

9.6%

6.5%

11.8%

10.4%

10.0%

2.65×

<0.001

eIF4A-like helicase

[PDB:PFB0445c]

[Genbank:AAC71878]

52.6

5.7

1

233

4

15%

0.99

1.05

1.24

1.22

7.9%

8.8%

15.7%

7.4%

8.3%

1.25×

0.024

    

2

368

7

26%

1.02

1.06

1.18

1.13

7.8%

4.2%

7.6%

7.3%

7.4%

1.16×

0.045

eIF5A

[PDB:PFL0210c]

[Genbank:AAN36131]

17.8

5.4

1

117

2

27%

0.12

0.32

1.26

1.79

18.2%

18.1%

10.9%

23.4%

18.2%

15.09×

<0.001

    

2

129

2

16%

1.05

0.98

0.79

0.55

4.9%

12.3%

30.1%

28.8%

20.5%

1.9×

0.005

    

3

151

2

16%

1.27

1.44

1.09

0.65

19.4%

24.0%

16.9%

21.6%

20.5%

2.23×

<0.001

Enolase

[PDB:PF10_0155]

[Genbank:AAN35353]

48.6

6.2

1

344

10

30%

1.10

1.11

1.07

0.88

4.2%

4.4%

4.6%

7.2%

4.5%

1.26×

<0.001

    

2

513

10

33%

1.15

1.18

0.99

0.83

14.1%

9.5%

4.8%

10.0%

9.8%

1.42×

<0.001

    

3

627

10

33%

1.18

1.17

1.09

0.87

5.4%

5.0%

7.9%

8.5%

6.7%

1.35×

<0.001

    

4

897

10

39%

1.13

1.06

1.11

0.92

11.7%

2.2%

8.3%

5.2%

6.7%

1.22×

0.016

    

5

138

4

15%

1.03

0.91

1.14

0.93

19.9%

7.8%

9.9%

11.9%

10.9%

1.25×

0.125

    

3a

213

5

14%

1.83

1.09

0.68

0.52

12.7%

12.9%

24.3%

3.3%

12.8%

3.52×

<0.001

    

4a

122

3

11%

1.43

1.06

0.85

0.73

0.9%

3.1%

16.0%

4.8%

4.0%

1.94×

<0.001

EXP-2

[PDB:PF14_0678]

[Genbank:AAN37291]

33.6

5.1

-

133

3

12%

0.27

0.70

0.88

0.97

8.1%

4.3%

10.4%

9.3%

8.7%

3.62×

<0.001

HSP40

[PDB:PFE0055c]

[PDB:MAL5P1.12]

[Genbank:CAD51377]

47.8

8.1

-

118

4

19%

0.70

0.85

0.94

2.06

13.2%

37.4%

14.2%

18.9%

16.5%

2.94×

<0.001

HSP60

[PDB:PF10_0153]

[Genbank:AAN35351]

62.9

6.7

1

171

5

11%

1.29

0.96

1.10

1.37

8.6%

4.1%

7.0%

3.8%

5.5%

1.43×

<0.001

    

2

235

5

11%

1.31

0.89

1.08

1.35

5.9%

3.8%

5.5%

6.5%

5.7%

1.51×

<0.001

HSP70-1a

[PDB:PF08_0054]

[Genbank:CAD51185]

74.4

5.5

1

607

10

20%

1.31

1.13

1.01

0.93

14.7%

10.1%

12.9%

7.9%

11.5%

1.41×

0.008

    

2

644

10

22%

1.46

1.23

1.06

0.96

5.7%

4.5%

10.8%

7.1%

6.4%

1.52×

<0.001

HSP70-2a

[PDB:PFI0875w]

[Genbank:CAD51861]

72.5

5.2

1

486

8

17%

0.95

1.15

0.98

1.08

8.7%

5.0%

7.8%

8.1%

8.0%

1.2×

0.014

    

2

724

10

23%

1.06

1.07

1.02

1.11

8.6%

4.8%

7.2%

4.4%

6.0%

1.08×

0.369

    

3

694

10

21%

1.26

0.87

1.03

1.09

4.9%

2.5%

6.1%

4.1%

4.5%

1.46×

<0.001

HSP70-3a

[PDB:PF11_0351]

[Genbank:AAN35935]

73.7

6.5

1

193

5

7%

1.08

0.97

0.97

1.06

7.2%

7.8%

16.2%

4.9%

7.5%

1.11×

0.299

    

2

488

8

12%

1.01

0.98

1.16

1.11

8.0%

2.2%

11.3%

6.2%

7.1%

1.19×

0.028

CA1

- [Genbank:NP_001729]

28.9

6.6

1

264

5

32%

1.48

0.98

0.60

0.59

6.2%

4.3%

13.7%

3.8%

5.2%

2.5×

<0.001

    

2

412

7

40%

1.16

0.94

0.72

0.77

6.0%

7.1%

17.4%

6.0%

6.6%

1.61×

<0.001

Human SOD1

- [Genbank:NP_000445]

16.2

5.7

-

247

5

57%

1.04

1.18

1.13

0.74

9.2%

12.5%

7.3%

16.2%

10.8%

1.6×

0.001

HETK

[PDB:PFF1335c]

[PDB:MAL6P1.153]

[Genbank:CAG25088]

20.6

7.0

-

575

8

56%

1.02

1.13

0.96

0.74

1.9%

9.0%

5.9%

7.5%

6.7%

1.53×

<0.001

Hypothetical protein PF10_0325

[PDB:PF10_0325]

[Genbank:AAN35522]

33.2

5.6

1

380

7

33%

1.08

1.11

1.04

0.93

1.5%

7.0%

11.7%

14.8%

9.4%

1.2×

0.107

    

2

497

8

33%

1.13

1.09

0.99

0.88

5.8%

4.9%

18.7%

17.9%

11.9%

1.28×

0.097

Hypothetical protein PFF1295w

[PDB:PFF1295w]

[Genbank:CAG25080]

43.9

7.2

-

245

5

20%

1.06

1.04

0.95

0.91

2.2%

7.1%

13.7%

5.0%

6.0%

1.16×

0.072

Hypothetical protein PFI1270w

[PDB:PFI1270w]

[Genbank:CAD51940]

24.9

5.5

1

332

6

21%

1.31

0.98

1.01

1.00

11.6%

8.7%

10.1%

14.3%

10.8%

1.34×

0.012

    

2

74

3

17%

0.93

0.97

1.36

1.65

8.6%

16.8%

11.6%

9.8%

10.7%

1.77×

<0.001

M1AP

[PDB:MAL13P1.56]

[Genbank:CAD52253]

126.6

7.3

-

171

7

10%

1.47

0.91

0.87

0.89

12.2%

8.7%

13.4%

10.9%

11.5%

1.69×

<0.001

OAT

[PDB:PFF0435w]

[PDB:MAL6P1.91]

46.0

6.5

-

589

9

29%

1.20

1.05

1.06

1.06

0.9%

5.8%

12.0%

5.4%

5.6%

1.14×

0.116

PEMT

[PDB:MAL13P1.214]

[Genbank:CAD52560]

31.3

5.4

1

790

9

46%

1.07

1.12

1.09

0.89

7.2%

10.3%

9.6%

16.7%

9.9%

1.26×

0.049

    

2

182

3

14%

0.76

0.97

1.13

1.05

6.2%

5.8%

14.0%

16.3%

10.1%

1.49×

0.003

Proteasome component C8

[PDB:PFC0745c]

[Genbank:CAB11152]

29.7

6.4

1

67

1

7%

1.17

1.20

1.12

0.83

12.9%

3.1%

5.1%

7.6%

6.4%

1.44×

<0.001

    

2

275

4

28%

0.98

0.96

0.96

0.82

7.4%

9.2%

10.8%

11.2%

10.0%

1.2×

0.072

TCP1a

[PDB:PF11_0331]

[Genbank:AAN35915]

60.6

6.7

-

110

5

11%

1.05

0.97

1.04

1.04

3.0%

4.1%

6.4%

6.2%

5.1%

1.08×

0.190

TPI

[PDB:PF14_0378]

[Genbank:AAN36991]

28.1

6.0

1

579

8

43%

1.18

1.13

1.04

0.86

6.1%

4.5%

11.8%

9.4%

7.7%

1.37×

<0.001

    

2

578

8

45%

1.20

1.11

1.07

0.88

7.0%

5.1%

10.0%

9.6%

8.3%

1.37×

<0.001

Uridine phosphorylase

[PDB:PFE0660c]

[Genbank:CAD51497]

27.5

6.1

-

430

7

35%

1.23

1.16

1.09

0.73

5.2%

3.6%

7.0%

6.5%

5.8%

1.68×

<0.001

  1. The average volume ratios represent log2-transformed values and have not been mean-centered around zero. aNomenclature in agreement with Shonhai and coworkers [35]. CA1, human carbonic anhydrase 1; eIF, eukaryotic initiation factor; EXP, exported protein; HETK, hydroxyethylthiazol kinase; HSP, heat shock protein; M1AP, M1-family aminopeptidase; OAT, ornithine aminotransferase; PEMT, phosphoethanolamine N-methyltransferase; SOD, superoxide dismutase; TCP1a, T-complex protein 1, α subunit; TPI, triose phosphate isomerase.
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Genome Biology

ISSN: 1474-760X

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