Figure 4

Direct interaction with Pax2 and predicted target genes. (a) Oligonucleotide sequences of the predicted target genes containing the PAX2/5/8 binding site. The consensus sequence is indicated in the top row in IUPAC code. Within this binding site, conserved base pairs between all the targets are highlighted in dark blue, and conserved base pairs in at least three targets genes are highlighted in light blue. The three point mutations in the PAX2/5/8 binding site of cldn7 are indicated in red. (b) Binding ability of Pax2 on the promoters of brn2, metrnl, sec31l and cldn7 in the presence of increasing concentrations of cold competitor. First lane: oligonucleotide only. Second lane: oligonucleotide + Pax2. Third lane: oligonucleotide + Pax2 + 100× cold competitor. Fourth lane: oligonucleotide + Pax2 + 500× cold competitor.