Figure 5
From: Germline mutagenesis mediated by Sleeping Beautytransposon system in mice
Possible explanation for why we could not detect GFP signal in double transgenic mice. Green fluorescent protein (GFP) expression at the donor site may be suppressed because of highly methylated transposon DNA (top panel). This suppression may be abrogated during germline transmission. However, the same suppression would be reintroduced at the donor site, probably because of the flanking sequence of the transposon DNA. The suppression may not be reintroduced into the transposed DNA (bottom panel).