Figure 4

Clusterin is highly expressed in the FVB/N strain. (a) qRT-PCR measurement of Clusterin RNA in prostate preparations used in microarray analysis. White bars are the data from the microarray experiments and black bars are values generated by qRT-PCR. (b) Western blot analysis of clusterin in the ventral prostates of FVB/N, DBA/2 and C57BL/6 mouse strains. Ventral prostate tissue (pool of three ventral prostates per lane/strain) was prepared and equal amounts of protein were resolved by SDS-PAGE and probed with anti-clusterin antibody. Antibody against β-actin was used as a loading control. (c, d) Immunohistochemical analysis of paraffin sections from dorsal prostate (DP), lateral prostate (LP), anterior prostate (AP) (c) and ventral prostate (VP) lobes (c, d) of 8-9 week old mice from FVB/N, DBA/2 and C57BL/6 strains. Sections were stained with anti-clusterin antibody. Clusterin immunoreactivity is most intense in the apical region of the secretory epithelial cells from the ventral prostate (arrow).