Figure 1
From: Genetic background influences murine prostate gene expression: implications for cancer phenotypes
Experimental design. Prostates from 12 mice from each of 5 strains of Mus musculus (C57BL/6, 129X1/Sv, BALB/c, FVB/N and DBA/2) were resected and individual lobes were dissected: DP, dorsal prostate; LP, lateral prostate; VP, ventral prostate; AP, anterior prostate. Each experimental sample represents a pool of equal amounts of RNA for each prostatic lobe from three animals. Four independent experimental samples were created per strain: 12 mice divided into 4 pools of 3 mice each for a total of 4 microarray experiments per strain. Amplified RNA from each experimental sample was hybridized against a reference pool onto custom mouse prostate cDNA microarrays using alternate dye-labeling to account for dye-specific effects.