Table 6 Primer sequences and PCR product sizes of genes selected for validation by QPCR
From: LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs
Gene | GenBank ID | Primer sequence | Annealing Tm (°C) | Product size (bp) |
|---|---|---|---|---|
H3F3A | NM_213930.1 | Forward 5'-GCAAGAGTGCGCCCTCTACT-3' Reverse 5'-TTGGCATAATTGTTACACGTTTGG-3' | 60 | 288 |
TNNC2 | NM_001001862.1 | Forward 5'-AAGGAGTTGGGCACCGTGAT-3' Reverse 5'-CGGCCTTCGTTGTTCTTGTC-3' | 60 | 326 |
MYL2 | NM_213791.1 | Forward 5'-GGGTGCTCAGGGCTGATTAT-3' Reverse 5'-AGGCTGCAAAGAAGATGAAGGT-3' | 60 | 326 |
MYL1 | NM_214374.1 | Forward 5'-GACTTTGTTGAGGGTCTGCG-3' Reverse 5'-GAGTGGTGCTTGGATTTGAG-3' | 60 | 452 |
SLN | Z98820.2 | Forward 5'-AGAATGGAGCGATCCACCCG-3' Reverse 5'-AAACACTTGGCAGCCCTTGA-3' | 60 | 300 |
MYLPF | NM_001006592.1 | Forward 5'-GAGAAGGGCAGCGGCAGAAG-3' Reverse 5'-GTGCGTGATGACGTAGCAGATGTT-3' | 60 | 466 |
TOB1 | EF486515 | Forward 5'-TTACCACTGCCACTTTCGCT-3' Reverse 5'-TTCTGCTTCAAGAGGTCATTCAC-3' | 61 | 129 |
CRABP1 | EF397416 | Forward 5'-GTGTGAACGCCATGCTGAG-3' Reverse 5'-CGTCCGTCCACTGTCTCC-3' | 55 | 169 |
LGALS1 | AY604429 | Forward 5'-GGTCGCCAGCAACCTGAATCTC-3' Reverse 5'-GTCTCCGTGCATGTCGAAGCG-3' | 58 | 151 |
GNB2L1 | NM_214332 | Forward 5'-GCTGGGACAAGCTGGTCAAGG-3' Reverse 5'-AGCACAGAGCCAGTAGCGATTG-3' | 58 | 245 |
TPT1 | NM_214373 | Forward 5'-GGCTGTTGGGATCGGATCTATC-3' Reverse 5'-AACAATGCCTCCGCTCCAAAG-3' | 55 | 150 |
RPS28 | NM_001001587 | Forward 5'-GGCAGGACAGGTTCGCAGG-3' Reverse 5'-ATATCCAGGACCCAGCCACAAC-3' | 56 | 179 |
TncRNA | EF397601 | Forward 5'-GACCGCTGTCGTCACTGTATG-3' Reverse 5'-AGCACTTGCCCAGCCCTAG-3' | 55 | 189 |