Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags

Table 3 Primers and PCR conditions

PigEST name	Gene symbol	Oligo sequence (5' to 3')	Amplicon length	Tm (°C)
Ss1.1-rhlv24b_a21.5	Troponin	CCAGAGTCCCCAGGATA TGAGCAGGGCTTTATTTGCATTC	100	63
Ss1.1-rcst01_n6.5	Pepsinogen A precursor	TACTGCTGCTCAGCTTG GTGTCCTTCAGCTTGCCATTCTT	106	60
Ss1.1-rcst21_l12.5	Pepsinogen C	TCCTGGTCCTTTTTGACACCTAGAGGACTTGCTGGGGTTG	108	60
Ss1.1-rhyp08c_e13.5.5	Myelin basic protein	GCAGGGCATAGAGATGGTGTCCCGACCCTGTTAGGAAGAT	100	60
Ss1.1-Liv1-LVRM1E040203.5.5	Fetuin B	GCCCTGTGTTTCAAATCCTGAGGAGCCACAAGGACAGCTA	100	60
Ss1.1-rnlu1830b_g11.5	SP-C	TGTACATCTAGGAAACATCAGATTCTTTGGTGGTAGAAGCC	201	60
Ss1.1-rill310b_f20.5	Gastrotropin	TGAACAGCCCCAACTACCACTCATGCCAGCTTCTTGCTTA	110	60
Ss1.1-rduo424b_g21.5	Vitamin D-dependent calcium binding protein	TGAGTGCCCAAAAGTCTCCTCAGTTGCTTCAGCTCCTCCT	153	60

Table of the used primers and their corresponding polymerase chain reaction (PCR) conditions for the eight selected genes for which quantitative PCR (qPCR) was carried out. The column 'PigEST name' indicates the name of contig. 'Gene name' is the gene name of the selected genes according to UniProt match. 'Oligo sequence (5' to 3')' is the oligonucleotide sequences used in the qPCR experiment. 'Amplicon length' is the length of the amplified product in the qPCR. 'Tm' indicates the annealing temperature used in the qPCR.

ISSN: 1474-760X