Figure 1From: Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9aComparison of histone H3 and H4 modifications in different cell types. (a) Purification of crude nucleosomal histones. Nucleosomal histones were separated on a 4-12% gradient NuPAGE gel and run in MES buffer (Invitrogen), fixed, and stained with Seeblue (Invitrogen). Lane 1, SeeBlue pre-stained molecular weight markers (Invitrogen); lane 2, nucleosomal histones from normal mouse liver; lane 3, nucleosomal histones from HeLa cells, purified on a POROS HQ column. (b) Methylation states of H3K9, H3K27, H3K36, and H4K20. nm, non-modified; me, monomethyl; me2, dimethyl; me3, trimethyl. Shown are the means of four independent experiments. (c) Basal amino-terminal modifications of histone 4 in the indicated cell types. 'H4 4-17nm': unmodified H4 peptide containing amino acids 4-17. Shown are the means of four independent experiments (± standard deviation). Pan-ac: panacetylated.Back to article page