Figure 5

Correlation between coding sequence (transcriptome) %GC3 and genome %GC for six nematode species with extensive available genomic sequence. The green line indicates the coding sequence %GC versus the full genomic %GC. In this case, coding sequence %GC3 is a contributor to the full genomic %GC such that X and Y are not independent variables. The red line indicates the coding sequence %GC3 versus noncoding genomic %GC. In this case, the coding sequence contribution has been removed from genomic totals such that X and Y are independent variables. For BM, TS, HC, and AC, the calculation of noncoding genomic %GC relies on the assumption that the species will have a similar breakdown of coding and noncoding sequence as CE. Assembly and gene calling for the BM, HC, TS, and AC sequences are needed to test this assumption. Definitions of species two letter codes are provided in Table 1.