Figure 1From: Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitorsApproach used for tear fluid analysis. The tear fluid was analyzed by both in-solution digestion (1 and 4 μl) and one-dimensional gel separation combined with MS (GeLC-MS; 2 lanes of 4 μl each) on a LTQ-FT, and also through GeLC-MS on a LTQ-Orbitrap. The numbers indicate the bands according to the slicing pattern used for sample fractionation prior to in situ digestion.Back to article page