Figure 4

Old friends: confirmation of microarray results. Real-time RT-PCR was performed for (a) FOXP3, (b) CTLA4, (c) CCR7, and RPS9 (data not shown) expression in MACS separated human CD4⁺CD25⁺ T_Reg and CD4⁺CD25^- naïve T cells. Following normalization to RPS9, relative mRNA amounts in CD4⁺CD25⁺ T_Reg cells were adjusted to corresponding expression levels in CD4⁺CD25^- naïve T cells and expressed as fold changes. Real-time RT-PCR results, indicated by black bars, were compared with fold changes arising from the Human T_Reg Chip (represented by grey bars). The healthy donors, randomly chosen, are specified by letters (see Table 1). RT-PCR, reverse transcription polymerase chain reaction.