Figure 3 From: A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones Verifying the exon trapping and mutagenesis approach for identifying distant branch points. The rat α-tropomyosin minigene (TS3St) and a derivative (ΔBP-175), in which the previously determined dBP of exon 3 had been mutated, and an additional mutant (ΔBP-175 -182) were transfected into HeLa cells. Splicing of transiently expressed RNA was analyzed by RT-PCR with a [32P]labeled primer in the PCR reaction. dBP, distant branch point; RT-PCR, reverse transcriptase polymerase chain reaction; WT, wild type.Back to article page