Figure 6

Characterization of a gene encoding Yfr6. (a) Sequence alignment of the region containing the transcriptional initiation site (TIS) and the first 97 transcribed nucleotides of Yfr6 from MED4 and SS120. The alignment begins with the TATA element (in red) preceding the mapped first transcribed guanidine (labelled by an arrow). (b) In Northern blots, a signal for the predicted Yfr6 was detected for 244 and 239 nucleotides in total RNA from MED4 (MED) and SS120 (SS1), respectively, but not in RNA from WH 8102 (WH8) and MIT 9313 (MIT). (c) Comparison of Yfr6 secondary structures using ConStruct version 3.0a [60] The base pairing probability is colour-coded from light yellow (low) to red (high). Missing positions are indicated by a dash. The predicted RNA structures were obtained by RNAfold at 24°C. Both sequences were equally weighted (1.0). The consensus was calculated based on the predicted optimum structures. Default parameters were used for all other options.