Correction: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

  • Steven L Salzberg1, 4Email author,

    Affiliated with

    • Julie C Dunning Hotopp1,

      Affiliated with

      • Arthur L Delcher1,

        Affiliated with

        • Mihai Pop1,

          Affiliated with

          • Douglas R Smith2,

            Affiliated with

            • Michael B Eisen3 and

              Affiliated with

              • William C Nelson1

                Affiliated with

                Genome Biology20056:402

                DOI: 10.1186/gb-2005-6-7-402

                Received: 10 May 2005

                Accepted: 27 May 2005

                Published: 24 June 2005

                 

                After the publication of this work [1], other researchers independently discovered that some of the data deposited in the NCBI Trace Archive was labeled erroneously. In particular, the sequencing center responsible for two of the Drosophila genome projects (Agencourt Biosciences) mistakenly deposited 20,000 sequences from D. ananassae and labeled them as D. mojavensis. The center recently corrected the mistake by removing the mislabeled sequences from the Trace Archive. We then searched through the newly updated D. mojavensis sequences for the 114 Wolbachia sequences that we had originally reported, and found that all had been removed. Thus our article should be corrected to report that new Wolbachia genome sequences were discovered in D. ananassae and D. simulans, but not in D. mojavensis.

                While searching the Trace Archive to verify this correction, however, one of us (S.L.S.) found that the traces for a new fly sequencing project, that of D. willistoni, had just been deposited. On searching the D. willistoni traces, a substantial Wolbachia infection in this species was discovered and 2,291 sequences belonging to Wolbachia were found. They were assembled into 485 contigs using the comparative assembler AMOS-Cmp [2] and the methods described in [1]. These sequences and assemblies are freely available for download from [3].

                Declarations

                Acknowledgements

                We thank Therese Markow of the University of Arizona for bringing this error in the Trace Archive data to our attention, and Jack Werren of the University of Rochester for suggesting that D. willistoni might have a Wolbachia infection.

                Authors’ Affiliations

                (1)
                The Institute for Genomic Research
                (2)
                Agencourt Bioscience Corporation
                (3)
                Center for Integrative Genomics, University of California
                (4)
                Center for Bioinformatics and Computational Biology, University of Maryland

                References

                1. Salzberg SL, Dunning Hotopp JC, Delcher AL, Pop M, Smith DR, Eisen MB, Nelson WC: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species. Genome Biol 2005, 6:R23.View ArticlePubMed
                2. Pop M, Phillippy A, Delcher AL, Salzberg SL: Comparative genome assembly. Brief Bioinform 2004, 5:237–248.View ArticlePubMed
                3. D. willistoni sequences and assemblies [ftp://​ftp.​cbcb.​umd.​edu/​pub/​salzberg]

                Copyright

                © BioMed Central Ltd 2005

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