Nonsense-mediated mRNA decay: from vacuum cleaner to Swiss army knife
© BioMed Central Ltd 2004
Published: 30 March 2004
Nonsense-mediated mRNA decay (NMD) downmodulates mRNAs that have in-frame premature termination codons and prevents translation of potentially harmful truncated proteins from aberrant mRNAs. Two new approaches have identified physiological NMD substrates, and suggest that NMD functions as a multipurpose tool in the modulation of gene expression.
Nonsense-mediated mRNA decay (NMD) is a specific pathway for the degradation of mRNAs that have premature termination codons (PTCs) in their open reading frames (ORFs). Its importance is highlighted by its conservation in all eukaryotes. NMD counteracts the potentially harmful impact of mRNAs that have PTCs as a result of errors at various levels of gene expression, such as nonsense and frameshift mutations, transcriptional errors and faulty splicing. Thus, NMD serves as a 'cellular vacuum cleaner' that protects the cell from the potentially harmful effects of truncated proteins by eliminating mRNAs with PTCs in a sequence of events that is not yet fully understood. In recent years numerous biochemical and cell-biological investigations in Saccharomyces cerevisiae , Drosophila melanogaster , Caenorhabditis elegans  and human [4, 5] cells have helped to elucidate some of the mechanistic details underlying the NMD pathway. A role for NMD in the regulation of mRNA metabolism beyond the mere vacuum cleaner function for faulty mRNAs has been suspected, and was foreshadowed by work on the splicing factor SC35 and some ribosomal proteins [6, 7]. Now, 'genome-wide' approaches - one in yeast using microarrays and another in silico, analyzing information mined from mRNA and protein databases - have added powerful evidence to suggest that NMD may serve multiple purposes in gene expression [8–11].
Inspirations from yeast
In yeast, NMD depends on the expression of the Upf1, Upf2 (Nmd2) and Upf3 proteins. Single or simultaneous inactivation of the UPF genes stabilizes nonsense-containing mRNAs, indicating that their protein products interact functionally in the same pathway. He et al.  used high-density oligonucleotide arrays to analyze genome-wide expression profiles of yeast strains containing single deletions of the UPF1, UPF2 or UPF3 genes, as well as of the DCP1 and XRN1 genes which encode proteins with activities thought to be involved in the NMD pathway - an essential component of the mRNA decapping enzyme and the 5'-3' exonuclease, respectively. They also tested double deletions of the XRN1 gene in combination with each of the UPF genes. Two-dimensional clustering analysis of the expressed genes for the Δupf1, Δupf2 and Δupf3 strains yielded several interesting results.
The deletion of UPF1, UPF2 or UPF3 generated nearly identical expression profiles. Thus, all three gene products act on the same targets, consistent with the function of Upf1, Upf2 and Upf3 in a single, linear pathway in yeast. The abundance of most mRNAs upregulated in Upf-deficient cells was also increased in Δdcp1 and Δxrn1 strains, suggesting that these mRNAs are largely degraded by decapping and subsequent 5'-3' exonucleolytic decay. This approach also identified a considerable number of NMD-regulated transcripts (765 out of the 7,839 genes represented on the microarrays) and showed that NMD substrates are generally expressed at below-average levels. In addition, most NMD substrates were found to be upregulated upon NMD inactivation, but some were downregulated, pointing to the existence of higher-order NMD targets (or additional functions of the Upf proteins in alternative gene-regulation pathways). Finally, only one third of the identified transcripts can be classified into structural or functional groups, some of which are surprising and hitherto unrecognized. Representatives of previously described NMD-substrate categories were identified, including mRNAs with nonsense mutations, transcripts resulting from faulty or regulated alternative splicing, mRNAs subject to leaky scanning during translation initiation, and mRNAs with an upstream ORF or with AATGA or ATGAA motifs immediately upstream of their translation initiation codons. More intriguing is the discovery of several new classes of NMD targets, including mRNAs that use translational +1 frameshifting, bicistronic mRNAs and, most interestingly, two classes of noncoding RNAs: pseudogene transcripts and transcripts encoded by transposable elements or their long terminal repeat (LTR) sequences.
In silico veritas?
In a series of three recent publications [9–11] Steve Brenner and colleagues suggested, by data-mining, that one-third of reliably inferred human splice products form a major class of natural targets for NMD. Alternative splicing is thought to occur in 30-60% of human genes, expanding the coding repertoire of the limited number of genes in the genome and modulating tissue-specific and developmental gene functions. Brenner and colleagues [9–11] now suggest that alternative splicing provides a mechanism to generate PTC-containing splice products that are subsequently degraded by NMD and, as a consequence, that cooperation between alternative splicing and NMD pathways offers a major and currently underappreciated way to regulate gene expression.
For their in silico analysis, they mapped well-characterized human RefSeq  and LocusLink  mRNA sequences to genomic sequences, and then performed high-stringency alignments between these 'RefSeq-coding genes' and expressed sequence tags (ESTs). The reliably inferred splice variants were only accepted as likely NMD targets when they conformed to the '50-nucleotide rule': this hallmark of mammalian NMD predicates that stop codons located at least 50 nucleotides upstream of the last exon junction will be interpreted as 'premature' and trigger NMD. This approach leads to an underestimate of potential NMD targets, because some PTCs (for example, in T-cell receptor gene transcripts) will trigger NMD even when they are not followed by a sufficiently distant intron . Moreover, Brenner and colleagues [9–11] also excluded mRNA variants that are indistinguishable from products of faulty splicing. These studies have unearthed several groups of functionally related proteins whose expression appears to be regulated by NMD, including translation factors and ribosomal proteins. This is in remarkable contrast to the yeast data of He et al. , where proteins with a function in translation were, if anything, underrepresented in the pool of NMD-regulated genes.
NMD is not (yet) on everyone's mind. As a consequence, Brenner and colleagues  found several entries for truncated proteins in the Swiss-Prot database. In some of these cases the available experimental evidence confirms that the mRNAs that encode these truncated proteins are bona fide NMD substrates. We are left with a consolation and a surprise. The consolation is that traces of NMD can be uncovered even though they had been overlooked before. Having recently had to accept that the number of genes in the human genome is too limited to explain the far higher number of proteins (not to mention other gene products), we then had to learn that one plausible and elegant explanation lies in alternative splicing, which enables a gene to code for a whole family of related, or sometimes antagonistic, proteins. And now the surprise is that a large portion of this effort is supposedly expended only to direct many of the primary products to decay. Several conclusions can be drawn from these observations. First, databases need to be read and annotated with a full realization of the implications of NMD; and second, NMD seems to serve as a tool for rapidly switching off gene expression. This view extends the idea of NMD as a mechanism for ridding the cell of the potentially harmful products of faulty splicing. But there may be more to it. NMD rarely downregulates the expression of a transcript completely; more commonly, 10-30% of the PTC-containing transcripts survive and may allow the production of physiologically relevant levels of truncated protein products.
For NMD researchers it has always been hard to reconcile these observations with the presumed protective role of NMD, especially as very low levels of biological products can sometimes have enormous effects. Given the problems of detecting the low levels of proteins or peptides produced from downregulated transcripts, in addition to some lingering lack of awareness of NMD, examples to prove otherwise are hard to come by. A recent publication  describes a PTC-containing transcript of the high-affinity immunoglobulin E (IgE) receptor, FcεRIβ, arising from retention of an intron. This alternative transcript not only conforms to the 50-nucleotide rule but its expression levels are very low compared to those of the full-length transcript, as would be expected for an NMD target. Nonetheless, the truncated protein is not only detectable, it even competes effectively with the full-length protein to control FcεRIβ expression on the cell surface. Thus, even low endogenous expression levels of NMD targets can suffice to generate a product with a biological function. A similar example of the utility of a bona fide NMD substrate is illustrated by the unc-49 locus in C. elegans. This locus uses alternative splicing to produce three GABA-receptor subunits, two of which (A and B) undergo several splice events in their 3' UTRs, rendering them predicted NMD substrates . While A-form transcripts are hardly detectable, B-form transcripts represent the most abundant form and code for a protein essential for the worm's locomotion. Either the B-form transcript escapes NMD, or the residual mRNA left after NMD suffices for the necessary protein production.
Among the examples presented by Brenner and colleagues  or in other studies  are genes with complex alternative splice patterns resulting in multiple transcript isoforms with or without PTCs. The lower abundance of these isoforms when compared to the full-length form supports the notion that they are targeted to the NMD pathway. But is the possibility that cells engage in complex alternative-splicing procedures generating multiple products, just to finally dispose of them, the only conceivable option? Four out of five PTC-containing isoforms of the mRNA for the LARD death receptor are readily detectable in non-activated lymphocytes, whereas only the full-length form is expressed in activated lymphocytes . While it is entirely possible that the PTC-containing forms are generated to switch off receptor expression in resting lymphocytes, there are attractive alternatives: as in the case of the FcεRIβ receptor , the PTC-containing mRNAs may produce proteins or peptides with relevant, although currently unknown, functions. Thus, quantitative control of the expression of low amounts of protein isoforms could represent yet another facet of the function of the NMD pathway.
A role for NMD in controlling the levels of noncoding RNAs (including noncoding alternative splice products) must also be considered. RNA accounts for more than 95% of the human genome's output and there is increasing evidence that noncoding RNAs (including introns, and spliced and polyadenylated transcripts) can have a function, for example as a modulating network or an additional layer of information [22, 23]. Importantly, noncoding RNAs have been discovered among natural NMD targets in yeast , where only a relatively small portion of the genome is transcribed into noncoding RNAs. What might be the role of NMD in mammals, which transcribe a far higher percentage of their genome into noncoding RNAs [22, 24, 25]? Clearly, the recent insights into the RNA-substrate spectrum of the NMD system should enhance the appreciation of NMD as a versatile, multipurpose mechanism that controls the transcriptome qualitatively and quantitatively.
- Gonzalez CI, Bhattacharya A, Wang W, Peltz SW: Nonsense-mediated mRNA decay inSaccharomyces cerevisiae.Gene 2001, 274:15–25.View ArticleGoogle Scholar
- Gatfield D, Unterholzner L, Ciccarelli FD, Bork P, Izaurralde E: Nonsense-mediated mRNA decay inDrosophila: at the intersection of the yeast and mammalian pathways.EMBO J 2003, 22:3960–3970.View ArticleGoogle Scholar
- Mango SE: Stop making nonSense: theC. eleganssmg genes.Trends Genet 2001, 17:646–653.View ArticleGoogle Scholar
- Schell T, Kulozik AE, Hentze MW: Integration of splicing, transport and translation to achieve mRNA quality control by the nonsense-mediated decay pathway.Genome Biol 2002, 3:reviews1006.1–1006.5.View ArticleGoogle Scholar
- Maquat L: Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics.Nat Rev Mol Cell Biol 2004, 5:89–99.View ArticleGoogle Scholar
- Sureau A, Gattoni R, Dooghe Y, Stevenin J, Soret J: SC35 autoregulates its expression by promoting splicing events that destabilize its mRNAs.EMBO J 2001, 20:1785–1796.View ArticleGoogle Scholar
- Mitrovich QM, Anderson P: Unproductively spliced ribosomal protein mRNAs are natural targets of mRNA surveillance inC. elegans.Genes Dev 2000, 14:2173–2184.View ArticleGoogle Scholar
- He F, Li X, Spatrick P, Casillo R, Dong S, Jacobson A: Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast.Mol Cell 2003, 12:1439–1452.View ArticleGoogle Scholar
- Lewis BP, Green RE, Brenner SE: Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans.Proc Natl Acad Sci USA 2003, 100:189–192.View ArticleGoogle Scholar
- Green RE, Lewis BP, Hillman RT, Blanchette M, Lareau LF, Garnett AT, Rio DC, Brenner SE: Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes.Bioinformatics 2003,19(Suppl 1):I118-I121.View ArticleGoogle Scholar
- Hillman RT, Green RE, Brenner SE: An unappreciated role for RNA surveillance.Genome Biol 2004, 5:R8.View ArticleGoogle Scholar
- Lelivelt MJ, Culbertson MR: Yeast Upf proteins required for RNA surveillance affect global expression of the yeast transcriptome.Mol Cell Biol 1999, 19:6710–6719.View ArticleGoogle Scholar
- Lew JE, Enomoto S, Berman J: Telomere length regulation and telomeric chromatin require the nonsense-mediated mRNA decay pathway.Mol Cell Biol 1998, 18:6121–6130.View ArticleGoogle Scholar
- Dahlseid JN, Lew-Smith J, Lelivelt MJ, Enomoto S, Ford A, Desruisseaux M, McClellan M, Lue N, Culbertson MR, Berman J: mRNAs encoding telomerase components and regulators are controlled by UPF genes inSaccharomyces cerevisiae.Eukaryot Cell 2003, 2:134–142.View ArticleGoogle Scholar
- Enomoto S, Glowczewski L, Lew-Smith J, Berman JG: Telomere cap components influence the rate of senescence in telomerase-deficient yeast cells.Mol Cell Biol 2004, 24:837–845.View ArticleGoogle Scholar
- NCBI Reference Sequence (RefSeq)[http://www.ncbi.nlm.nih.gov/RefSeq/]
- Carter MS, Li S, Wilkinson MF: A splicing-dependent regulatory mechanism that detects translation signals.EMBO J 1996, 15:5965–5975.Google Scholar
- Donnadieu E, Jouvin MH, Rana S, Moffatt MF, Mockford EH, Cookson WO, Kinet JP: Competing functions encoded in the allergy-associated F(c)epsilonRIbeta gene.Immunity 2003, 18:665–674.View ArticleGoogle Scholar
- Bamber BA, Beg AA, Twyman RE, Jorgensen EM: TheCaenorhabditis elegansunc-49 locus encodes multiple subunits of a heteromultimeric GABA receptor.J Neurosci 1999, 19:5348–5359.Google Scholar
- Wollerton MC, Gooding C, Wagner EJ, Garcia-Blanco MA, Smith CW: Autoregulation of polypyrimidine tract binding protein by alternative splicing leading to nonsense-mediated decay.Mol Cell 2004, 13:91–100.View ArticleGoogle Scholar
- Mattick JS, Gagen MJ: The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms.Mol Biol Evol 2001, 18:1611–1630.View ArticleGoogle Scholar
- Mattick JS: Challenging the dogma: the hidden layer of non-protein-coding RNAs in complex organisms.Bioessays 2003, 25:930–939.View ArticleGoogle Scholar
- Eddy SR: Noncoding RNA genes.Curr Opin Genet Dev 1999, 9:695–699.View ArticleGoogle Scholar
- Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, et al.: The sequence of the human genome.Science 2001, 291:1304–1351.View ArticleGoogle Scholar