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Table 4 Primers used to construct the GFP promoter fusions

From: In silico identification and experimental validation of PmrAB targets in Salmonella typhimuriumby regulatory motif detection

Name

Sequence 5' to 3'

Description

Amplification of promoter regions

Pro-115

CC GAATTC TAATTCGAGTTGCTTAAAGGCGGC

Amplification of yibD promoter region

Pro-116

CC GGATCC GCTCCCGCATTATATAACGGG

Amplification of yibD promoter region

Pro-117

CC GAATTC GCCAATAAAAACCGCGCAGAGTG

Amplification of mig-13 promoter region

Pro-118

CC GGATCC AGCGAGTTGTTAAGGTTTTCCAGC

Amplification of mig-13promoter region

Pro-119

CC GAATTC GAAGATTCCGCAGAATCAACGGCC

Amplification of aroQ promoter region

Pro-120

CC GGATCC GGTGCTGCACATCAATAAAGAACAAAG

Amplification of aroQ promoter region

Pro-121

CC GAATTC GTATTGCATCTGGGCGGTCATCG

Amplification of pmrC promoter region

Pro-122

CC GGATCC AGGCGATTTGCCCAAGAACAGG

Amplification of pmrC promoter region

Pro-224

AT GAATTC GCTTCCCCATCCCAAACCACC

Amplification of sseJ promoter region

Pro-225

AT GGATCC GGAAGGCGTGCGCTTTCTTTTATC

Amplification of sseJ promoter region

  1. Restriction sites in the primers are indicated in bold type.