Figure 3

Site-directed mutagenesis of the PmrA box in yibD. (a) Construction of six species of the yibD promoter mutant, designated pCMPG5615 to pCMPG5620, each with a single base substitution (T → G or A → C) in the PmrA box. Promoters were fused to GFP and promoter activity was assessed by FACS analysis. (b) Plot of the normalized expression values of the six mutant fusions and the wild-type fusion measured in two distinct conditions in the wild type and pmrA::Tn10d mutant background. Gray bars represent condition 1 (pH 7.7, 100 μM FeCl₃ + 10 μM MgCl₂), white bars correspond to the expression values observed in condition 2 (pH 7.7, 100 μM FeCl₃ + 10 mM MgCl₂). w, wild-type background; m, pmrA::Tn10d mutant background. The pmrC::GFP fusion was included as a positive control. Bars represent the standard deviations of three independent measurements.