Figure 3
From: Assembly and characterization of heterochromatin and euchromatin on human artificial chromosomes
Transcriptionally competent chromatin is present on artificial chromosomes. Dimethylation of lysine 4 on histone H3 (H3DimK4) was visualized using an antibody against H3DimK4 (red). This euchromatin mark was detected on all artificial chromosomes (arrowheads) generated from either D17Z1 in lines (a) 17-D34, (b) 17-E29, (c) 17-B12 and (d) 17-C20, or DXZ1 in lines (e) X-4 or (f) X-5. Host centromere regions were generally depleted for H3DimK4 as indicated by arrows pointing to centromere regions of chromosome 17 (a-d) and the X chromosome (e, f).