MicroRNA targets in Drosophila

Table 2 Whole genome comparison of real versus randomized miRNAs against the complete genomes of D. melanogaster and D. pseudoobscura

	Total hits	Total conserved hits	1 site	≥ 2 sites
73 D. melanogaster miRNAs (A)	6,864	589	556	33
73 Random miRNAs (B)	5,152	204	201	3
Standard deviation (100 experiments)	± 132	± 43	± 40	± 3
Ratio (A/B)		2.9	2.8	11.0
Estimated false positives (%)		35%	36%	9%

Detected conserved hits (especially those with multiple detected sites in the 3' UTR) are significantly over-represented (2.8× and 11× as many cases, on average, respectively) in analyses with actual miRNAs compared to randomly shuffled miRNAs. The thresholds used for this analysis were S: 100; ΔG: -19 kcal/mol; ID: 70%.

ISSN: 1474-760X