Figure 1
From: Characterizing the stress/defense transcriptome of Arabidopsis
Analysis of subtraction efficiency using PCR. Tester cDNA was prepared from the poly(A)+ RNA of plants sprayed with the virulent oomycete P. parasitica and the driver cDNA was from water-treated control plants. The unsubtracted and subtracted pools of cDNA were amplified using primers for the pathogen-inducible PR1 gene or the constitutively expressed G3PDH gene. Aliquots of the samples were taken after 15, 20, 25 and 30 cycles of PCR amplification and the products were analyzed on a 2% agarose gel.