Figure 3

Northern analysis of PGMsense and PGMinvrpt construct lines. Total RNA was isolated from adult males of the indicated PGMsense lines, PGMinvrpt lines, and control line, with or without DOX treatment. (a) Northern blot hybridized with a probe that will recognize both endogenous and transgenic Pgm transcripts and with a probe specific for Rp49 as a loading control. (b) Northern blot hybridized with a probe that will recognize only the endogenous Pgm transcript and with a probe specific for Rp49 as a loading control. The fold decrease in Pgm transcript abundance is indicated below the lanes. (c) Northern blot of control strain hybridized with a probe that will recognize the endogenous Pgm transcript and with a probe specific for Rp49 as a loading control.