Table 4 Primer and probe sequences used for RT-PCR (Taqman)
From: Microarray profile of differentially expressed genes in a monkey model of allergic asthma
Gene | Forward primer | Reverse primer | Probe | Species | Accession number |
|---|---|---|---|---|---|
α1-Antichymotrypsin | GACAGGTTCACGGAGGATGC | TGAGTCCTGAAAGTCAGTGGCA | AAGAGGCTGTATGGCTCCGAGGCC | Human | K01500 |
Calcitonin gene-related peptide receptor-1 | GATTCCATGGCGACCTGAAG | AGAGACCAAAAGACCCTGGAAGT | CAGAGGAGGTATATGACTACATCATGCACATCCTTATG | Human | L76380 |
Chitotriosidase | GACCCTGTTAGCCATCGGAG | GTTGGCCGTGGCTACCATA | CTGGAATTTCGGCACTCAGAAGTTCACAG | Human | U29615 |
DMBT1 | TTGAAGGTGGCTGCAACTATGATTAT | CAAACTCGAGCAATGAGAGGG | TTTCGATGGCCCCTACCGCAGTT | Monkey | AJ000342 |
Fibronectin | AATGTTCAGCTCACTGGATATCGA | AGTCCTGATACAACCACGGATGA | AGAAGACCGGACCAATGAAAGAAATCAACCT | Human | XM_055254 |
ICAM-1 | GGAGCTTCGTGTCCTGTATGG | GAATTTTCTGGCCACGTCCA | CCCCGACTGGACGAGAGGGATTGT | Human | NM_000201 |
Integrin alpha 6 | TGATCGAAATTCCTACCCTGATG | TAATCACAGGCCGGGATCTG | TGCTGTTGGTTCCCTCTCAGATTCAGTAACTATTC | Human | X59512 |
MCP-1 | GCTGTGATCTTCAAGACCATTGTG | TGGAATCCTGAACCCACTTCTG | CCAAGGAGATCTGTGCTGACCCCAA | Monkey | AF276081 |
MCP-3 | GAGCTACAGAAGGACCACCAGT | AAGTCCTGGACCCACTTCTG | TCCCCGGGAAGCTGTAATCTTCAAGA | Human | X71087 |
Mn superoxide dismutase | GGCCTACGTGAACAACCTGAAC | CTGTAACATCTCCCTTGGCCA | TCACCGAGGAGAAGTACCAGGAGGCG | Human | Y00497 |
N-acetylgalactosaminyl-transferase-2 | TGGCAGTGGCACTGTCTTTG | TTTGTATTCATCCATCCAGACCTC | CGAAACACCCGCCGGGCAGC | Human | NM_004481 |
Orphan nuclear hormone receptor BD73 | GAGGTGTGATTGCCTATATCAGTTCTT | TCTCAGAACCCTCACTGTGACAA | CAGCTCAGCCTCAAGCCCTGCCT | Human | L31785 |
PARC | TCTGTGCTGACCCCAATAAGAA | TCCAGGCCCCTCAGGC | TGGGTCCAGAAATACATCAGCGACCTG | Human | AB000221 |
Pepsinogen C | AAGAGTTTCTCATTGGCGGC | CCTGTATCCACGATAGCCTGG | CCTCCGGCTGGTGTTCTGAGGGTT | Monkey | X59754 |
Plasminogen activator inhibitor-1 | TCTTCGTCCAGCGGGATC | GTGCTCCGGAACAGCCTG | AAGCTGGTCCAGGGCTTCATGCC | Human | M14083 |
Selenoprotein-P | CAGTGACTGTGGTTGCTCTT | CAGTTTTACTCGCAGGTCTTC | CTTCAAGCCAGCTGATACCTGTG | Human | Z11793 |
SM22 | GGTGAAGGTGCCCGAGAA | AGGAACTGAGCCACCTGCTC | CCACCCTCCATGGTCTTCAAGCAGA | Human | AF013711 |
Thrombospondin | CATCCGCAAAGTGACTGAAGAG | CTGTACTGAACTCCGTTGTGATAGC | CCAATGAGCTGAGGCGGCCTCC | Human | X14787 |
TIMP1 | CACCCACAGACGGCCTTCT | AGGCTTGGAACCCTTTATACATCTT | TCAACCAGACCACCTTATACCAGCGTTATGA | Human | M12670 |
Eotaxin | CAT#: 4312354 (PE Biosystems, Foster City, CA.) | Â | Â | Human | Â |