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Table 5 Kinetic constants, pI and Mw of *AroQ enzymes

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

   

pI/Mr

Organism

Km (μM)

kcat (s-1)

Uncleaved

Cleaved

Pseudomonas

98

6.4

8.77 / 20,417

8.04 / 17,939

aeruginosa

    

Salmonella

142

8.9

7.02 / 20,646

6.58 / 18,392

typhimurium

    

Erwinia

169

9.7

6.28 / 20,299

6.24 / 18,017

herbicola

    

Yersinia

  

6.43 / 20,761

5.66 / 17,504

pestis

    

Mycobacterium

  

5.40 / 21,945

5.03 / 18,475

tuberculosis

    

Meloidogyne

  

6.58 / 22,087

5.90 / 19,460

javanica

    
  1. Reaction kinetics were monitored at 32°C using 40–75 nM enzyme purified in this study (P. aeruginosa and S. typhimurium) or previously (E. herbicola [12]). Bovine serum albumin was present at 0.1 mg/ml. Initial rates were used to calculate the steady-state kinetic parameters of Km and kcat. pI/Mr valueswere determined by use of the compute pI/Mw tool at ExPASy [47].
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Genome Biology

ISSN: 1474-760X

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