Figure 2

Southern blot analysis of EL702 transgenic Arabidopsis. Transformed Arabidopsis lines were characterized by Southern blot analysis and two independent lines identified. Line EL702C contains three copies of the inserts with near full-length LacOs (10.1 kb, approximately 250mer) incorporated into the genome. Line EL702E contains a single copy of LacOs with about half the number of binding sites (approximately 4.5 kb, approximately 120mer) incorporated into the genome. (a,b) Genomic DNA (20 μg) was digested with restriction enzymes and blotted onto nitrocellulose membranes after electrophoresis in a 0.7% agarose gel. The membranes were hybridized with (a) the lac operator and (b) the LacI/NLS probes described in Figure 1a. M, 1 kb size marker. B, BglII; P, PstI; H, HindIII.