Figure 5
From: Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25
Deubiquitinating activity assay for USP25, the muscular USP25 isoform and USP28. The immunoblot was detected using anti-β-galactosidase (β-gal) antibodies. The sizes corresponding to Ub-β-gal, β-gal and endogenous deleted β-gal are shown by black arrows. Arrowheads on the lanes show the size of the Ub-β-gal fusion protein and the deubiquitinated β-gal. Lane 1, molecular weight marker containing wild-type β-gal; lane 2, untransformed XL1blue (negative control); lanes 3 and 4, XL1blue cells transformed, respectively, with pAC-M-β-gal alone or pAC-M-β-gal and empty pGEX vector; lanes 5, 6 and 7, XL1blue cells transformed, respectively, with pAC-M-β-gal and USP25, pAC-M-β-gal and muscular USP25 isoform, and pAC-M-β-gal and USP28; lane 8, XL1blue cells transformed with the empty pGEX vector.