Skip to main content
  • Research news
  • Published:

Transfecting with antibodies

The various available transfection methods suffer from low efficiency, lack of specificity for a particular cell type, and a tendency to kill many of the target cells. Bildirici et al. offer a solution in the 18 May Nature, using antibody-coated beads (Nature 2000, 405:298). DNA enters the cells after agitation of a bead-cell mixture tears holes in the cell membrane. Transfection efficiency ranges from 40 to 80% with less than 20% cell killing, and the DNA is targeted to cells with the relevant cell surface marker. This targeting will be particularly important for ex vivo gene therapy applications.

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wells, W. Transfecting with antibodies. Genome Biol 1, spotlight-20000523-02 (2000). https://doi.org/10.1186/gb-spotlight-20000523-02

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/gb-spotlight-20000523-02

Keywords